Short-range synthetic aperture imaging at 633 nm by digital holography

We present an active optical synthetic aperture-imaging system. A phase-step digital holographic setup is used as a wavefront sensor in the far field. The overlap of the holograms enables the estimation and compensation of their relative positions and phase with a speckle cross-correlation algorithm. Experimental results on a short-range synthetic aperture setup at 633 nm are presented that are based on 128 x 128 holograms. The synthesis is executed in one direction by means of rotation of the object. Test images show a significant gain of resolution in the synthesis direction. Processing errors are estimated through experiment. Random processing errors of a synthetic pupil composed of 33 merged holograms are negligible, but biases induced by unknown optical aberrations ofthe reference wave induce defocusing and astigmatism.     

Update strategies for kriging models used in variable fidelity optimization

Many optimization methods for simulation-based design rely on the sequential use of metamodels to reduce the associated computational burden. In particular, kriging models are frequently used in variable fidelity optimization. Nevertheless, such methods may become computationally inefficient when solving problems with large numbers of design variables and/or sampled data points due to the expensive process of optimizing the kriging model parameters in each iteration. One solution to this problem would be to replace the kriging models with traditional Taylor series response surface models. Kriging models, however, were shown to provide good approximations of computer simulations that incorporate larger amounts of data, resulting in better global accuracy. In this paper, a metamodel update management scheme (MUMS) is proposed to reduce the cost of using kriging models sequentially by updating the kriging model parameters only when they produce a poor approximation. The scheme uses the trust region ratio (TR-MUMS), which is a ratio that compares the approximation to the true model. Two demonstration problems are used to evaluate the proposed method: an internal combustion engine sizing problem and a control-augmented structural design problem. The results indicate that the TR-MUMS approach is very effective; on the demonstration problems, it reduced the number of likelihood evaluations by three orders of magnitude compared to using a global optimizer to find the kriging parameters in every iteration. It was also found that in trust region-based method, the kriging model parameters need not be updated using a global optimizer—local methods perform just as well in terms of providing a good approximation without affecting the overall convergence rate, which, in turn, results in a faster execution time.     

Detection of Defects Using Fault Model Oriented Test Sequences

This paper analyzes the possibilities and limitations of defect detection using fault model oriented test sequences. The analysis is conducted through the example of a short defect considering the static voltage test technique. Firstly, the problem of defect excitation and effect propagation is studied. It is shown that the effect can be either a defective effect or a defect-free effect depending on the value of unpredictable parameters. The concept of Analog Detectability Interval (ADI) is used to represent the range of the unpredictable parameters creating a defective effect. It is demonstrated that the ADIs are pattern dependent. New concepts (Global ADI, Covered ADI) are then proposed to optimize the defect detection taking into account the unpredictable parameters. Finally, the ability of a fault oriented test sequence to detect defect is discussed. In particular, it is shown that the test sequence generated to target the stuck-at faults can reasonably guarantee short defect detection till a limit given by the Analog Detectability Intervals.     

Cocontraction changes in muscular fatigue at different levels of isometric contraction

Ten normal subjects participated in a study designed to contrast results obtained in pre- and post-fatigue states. The measures contrasted were the IEMG ratios of agonist/antagonist pairs of muscles. The experimental task was an 8 s ramp isometric elbow flexion ranging from 0 to 100% of a maximal voluntary contraction (MVC). IEMG ratios were obtained at levels of 20, 40, 60 and 80% MVC. Records from the following muscles were obtained with surface electrodes: biceps brachii (BB), brachioradialis (BR), triceps brachii (TB) and anconeus (SU). The torque at the elbow joint was measured by a Cybex II dynamometer. Fatigue was induced using a 60% MVC of elbow flexion maintained during 5 minutes. The data were collected on-line at a sampling rate of 1 kHz. The results indicated that the IEMG ratios (BB/TB and BR/SU) presented a tendency, across subjects, toward a decrease at the levels of 40%, 60% and 80% MVC at the post- relative to the pre-fatigue state. The BB/BR ratios remained stable. These changes in the post-fatigue ratios disclose a tendency toward a saturation of the agonist occurring concomitantly with an increased level of contraction of the antagonists.     

In vivo spatio-temporal visualization of the human skin by real-time confocal microscopy

A modified tandem scanning confocal microscope is used to obtain in vivo images of the human skin in real time. Three-dimensional and temporal visualizations are demonstrated with volume reconstruction and blood flow images. Two image processing methods based on Fourier transform and logarithmic processing are presented. Their applications in noise removal of the scanning disk lines and of the heterogeneity of light are illustrated.     

Effects of neuromuscular blocking agents on excitatory transmission and gamma-aminobutyric acidA-mediated inhibition in the rat hippocampal slice

BACKGROUND: Although neuromuscular blocking agents do not cross the blood-brain barrier, they may penetrate the central nervous system under particular circumstances and eventually cause neurotoxic consequences. METHODS: The effects of neuromuscular blocking agents on excitatory and inhibitory transmission in area CA1 of rat hippocampal slices were investigated using extracellular and intracellular recording techniques. RESULTS: Application of atracurium in the perfusion medium resulted in a dose-dependent enhancement of excitatory synaptic responses averaging 48.7 +/- 4.3% at a concentration of 10 nM. This effect was correlated with an increase in the size of the presynaptic fiber volley. Laudanosine, but not pancuronium bromide or vecuronium bromide, produced similar changes. In addition, atracurium and laudanosine blocked inhibitory transmission and reduced intracellularly recorded gamma-aminobutyric acidA receptor-mediated potentials. These effects were observed only at concentrations >1 microM and were not reproduced by pancuronium bromide and vecuronium bromide. CONCLUSIONS: Atracurium and its metabolite, laudanosine, contrary to pancuronium bromide and vecuronium bromide, produce two distinct effects on hippocampal slices. They enhance excitatory transmission and neuronal excitability and they block inhibitory gamma-aminobutyric acidA-mediated synaptic responses.     

Solution- and crystal-phase covalent modification of lysozyme by a purpose-designed organoruthenium complex. A MALDI-TOF MS study of its metal binding sites

Study of the reaction between the transition organometallic complex 4-ruthenocenyl 2,6-dimethylpyrylium tetrafluoroborate and the enzyme hen egg white lysozyme (HEWL) in solution and by diffusion in crystals was performed by use of a combination of spectroscopic and chromatographic methods. Conjugation involving the lysine residues of lysozyme appeared to occur readily, yielding very stable ruthenocenyl pyridinium adducts with average degrees of incorporation ranging from 0.2 to 1.8 metal complexes per protein molecule, depending on reaction conditions. Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) revealed that the protein conjugates were in fact mixtures of unmodified, mono-, di- and sometimes tripyridinium adducts. In combination with reversed-phased HPLC, we were able to show that six different monoruthenocenyl pyridinium adducts were formed in solution. This result was confirmed by trypsin digestion of a ruthenocenyl pyridinium conjugate and MALDI-TOF MS analysis of the peptide mixture, which showed that lysines 1, 13, 33, 96, 97 and 116 were involved in the reaction with the pyrylium complex, lysines 13, 33 and 116 being the major binding sites. In the tetragonal crystal state, no binding of the ruthenium complex was shown to occur at lysine 116, owing to steric hindrance at this particular position.     

Near-field optical patterning and structuring based on local-field enhancement at the extremity of a metal tip

We present a particular approach and the associated results allowing the nanostructuration of a thin photosensitive polymer film. This approach based on a scanning near-field optical microscopy configuration uses the field-enhancement (FE) effect, a so-called lightning-rod effect appearing at the extremity of a metallic tip when illuminated with an incident light polarized along the tip axis. The local enhancement of the electromagnetic field straight below the tip's apex is observed directly through a photoisomerization reaction, inducing the growth of a topographical nanodot characterized in situ by atomic-force microscopy using the same probe. From a survey of the literature, we first review the different experimental approaches offered to nanostructure materials by near-field optical techniques. We describe more particularly the FE effect approach. An overview of the theoretical approach of this effect is then given before presenting some experimental results so as theoretical results using the finite-element method. These results show the influence on the nanostructuration of the polymer of a few experimental parameters such as the polarization state, the illumination mode and the tip's geometry. Finally, the potentiality of this technique for some applications in the field of lithography and high-density data storage is shown via the fabrication of nano-patterns.     

Design and implementation of software for assembly and browsing of 3D brain atlases

Visualization software for three dimensional digital brain atlases present many challenges in design and implementation. These challenges include the design of an effective human interface, management of large data sets, display speed when slicing the data set for viewing/browsing, and the display of delineated volumes of interest (VOI). We present a software design, implementation and storage architecture that addresses these issues, allowing the user to navigate through a reconstructed volume quickly and smoothly, with an easy-to-use human interface. The software (macostat, for use with Macintosh OS) allows the user to rapidly display slices of the digital atlas at any arbitrary slicing angle, complete with delineated VOIs. The VOIs can be assigned colors of the user's choosing. The entire atlas, or selected portions, may be resliced with slices stored as individual image files, complete with delineations. These delineations may be transferred to corresponding sections of experimental materials using our analysis program (brain). The software may be obtained from the laboratory's web site: http://www.neuroterrain.org